| Conditions of enzyme activity |
Enzyme parameters |
Specific activity (U/mg) 3 h |
| Metal ion cofactors(1.0 mM) |
Lead, tin andcadmium |
527.8, 305.6 and 194.4 |
| Optimum metal ion cofactor |
lead |
|
| Range of lead ion concentration tested |
0.5-5.0 mM |
|
| Optimum lead ion concentration |
1.2 mM |
590.7 |
| Range of temperature |
10°C-80°C |
|
| Optimum temperature |
22°C |
482.2 |
| Range of pH |
02-Nov |
|
| Optimum pH |
3.5 and 8.5 |
322.2 and 344.4 |
| Substrates |
Mustard oil, coconut oil,
sunflower oil, olive oil, tributyrin |
86.66, 91.11, 71.11, 113.33 and 106.66 |
| Optimum substrate |
Olive oil |
113.33 |
| Rate when olive oil is the substrate |
Vmax olive oil |
144.93U/mg/min |
| Km when olive oil is the substrate |
Km olive oil |
0.260 mM |
