Purification steps Totalenzyme(U/L) Total protein(mg) Specific activity(U/mg) Foldpurification Enzyme Yield (%)
Crude extract 4,45,000 900 494.4 1.0 100
Ammonium sulphate precipitation (80%) 2,86,760 200 1433.8 2.9 64.4
Acetone precipitation (30%) 4,74,624 80 593.28 1.2 10.6
Table 2: Purification process of lipase from the psychrotrophic bacterium Pseudomonas sp. ADT3.