Samples Hydro carbon/oil Cell-free medium Culture medium  Emulsifying indexcell (%)a Emulsifying indexcell (%)a E24 E48 E72 E96 E24 E48 E72 E96 B. elkaniiSEMIA 587 Soybean oil 65.22 53.33 37.78 26.67 79.17 57.17 54.17 37.5   Sunflower oil 41.06 43.75 48.5 40.38 95 50 50 31.25   Diesel oil 34.09 31.82 27.27 25 --- --- --- --- 587::TnphoA24 Soybean oil 88.64 79.07 72.09 62.79 55.83 79.47 79.17 54.17   Sunflower oil 51.02 51.15 49.56 42.89 60.83 45.83 46 41.87   Diesel oil 71.74 71.74 65.22 64.44 --- --- --- --- 587::TnphoA31 Soybean oil 72.34 69.57 63.04 60.87 62.5 40 40,0 35.83   Sunflower oil 51.5 50 53.33 51.78 58.33 59.17 54.17 37.5   Diesel oil 74.42 75 65.91 58.14 --- ---- ---- ---- 587::TnphoA33 Soybean oil 58.7 52.17 50 50 54.14 45,83 45,83 38,17   Sunflower oil 51.92 50.22 45.78 58.25 87.5 87,50 79,85 29,17   Diesel oil 55.56 51.11 51.11 48.89 --- --- --- ---- 587::TnphoA50 Soybean oil 47.83 53.19 46.81 43.48 79.17 37,50 37,50 37,50   Sunflower oil 50 43.27 39.02 45 87.5 87,50 65,00 43,53   Diesel oil 58.14 55.81 51.16 52.27 --- --- --- ---

aResults are expressed as percentages of the total height occupied by the emulsion; values are means of at least three determinations -- Diesel oil not emulsified with cell culture

Table 1: Stability of emulsifying activity of culture medium and a substantially cell-free medium from a bacterial cell culture in which wild-type SEMIA 587 or mutant strains were grown