Dose (mg/kg) |
At 100nm Exposure |
At 50nm Exposure |
At 35nm Exposure |
At 10nm Exposure |
SOD |
LPX |
SOD |
LPX |
SOD |
LPX |
SOD |
LPX |
0 |
0.16 ± 0.08* |
5.28 ± 0.08 |
0.16 ± 0.04 |
5.14 ± 0.46 |
0.16 ± 0.07 |
5.37 ± 0.22 |
0.16 ± 0.02 |
6.07 ± 0.38 |
0.5 |
0.16 ± 0.02 |
5.56 ± 0.18 |
0.16 ± 0.07 |
6.05 ± 0.48 |
0.16 ± 0.0*2 |
6.36 ± 0.78 |
0.16 ± 0.06* |
6.16 ± 0.84 |
1.0 |
0.16 ± 0.08* |
5.08 ± 0.08* |
0.16 ± 0.08 |
6.13 ± 1.06 |
0.16 ± 0.08 |
6.11 ± 0.16* |
0.16 ± 0.04 |
6.25 ± 0.4* |
1.5 |
0.16 ± 0.10 |
5.79 ± 0.88 |
0.16 ± 0.08 |
6.06 ± 0.42 |
0.16 ± 0.08 |
6.34 ± 0.88 |
0.16 ± 0.08 |
6.10 ± 0.22 |
2.0 |
0.12 ± 0.08* |
5.58 ± 0.55 |
0.15 ± 0.08 |
5.89 ± 0.36* |
0.16 ± 0.02 |
6.26 ± 0.34 |
0.16 ± 0.06 |
6.69 ± 0.94 |
2.5 |
0.16 ± 0.10 |
5.30 ± 0.58* |
0.16 ± 0.06 |
6.25 ± 0.38 |
0.16 ± 0.06 |
6.18 ± 0.76 |
0.16 ± 0.01* |
7.15 ± 0.46 |
3.0 |
0.16 ± 0.22* |
4.84 ± 0.12 |
0.16 ± 0.06 |
6.43 ± 0.64 |
0.16 ± 0.04* |
6.37 ± 0.72 |
0.15 ± 0.10 |
7.22 ± 0.71 |
3.5 |
0.22 ± 0.08* |
5.56 ± 18 |
0.16 ± 0.04 |
7.05 ± 1.04 |
0.16 ± 0.02 |
6.11 ± 0.12 |
0.15 ± 0.04 |
7.59 ± 0.04* |
4.0 |
0.15 ± 0.0*7 |
5.93 ± 0.14 |
0.16 ± 0.06 |
7.02 ± 0.12 |
0.16 ± 0.04 |
6.23 ± 0.67* |
0.15 ± 0.02 |
8.07 ± 0.42 |
4.5 |
0.15 ± 0.08 |
5.79 ± 0.75 |
0.16 ± 0.06 |
6.09 ± 0.28 |
0.15 ± 0.06 |
6.38 ± 0.68 |
0.15 ± 0.04 |
7.89 ± 0.42 |
5.0 |
0.15 ± 0.08* |
5.54 ± 0.28 |
0.16 ± 0.06 |
6.78 ± 0.5 |
0.15 ± 0.08 |
7.29 ± 0.17 |
0.15 ± 0.04* |
8.03 ± 0.62 |
5.5 |
0.15 ± 0.08 |
5.56 ± 0.18 |
0.16 ± 0.06 |
5.89 ± 0.38 |
0.15 ± 0.07 |
7.39 ± 0.18 |
0.15 ± 0.08 |
8.12 ± 0.18 |
6.0 |
0.15 ± 0.12 |
5.94 ± 0.20 |
0.16 ± 0.12 |
6.45 ± 0.32 |
0.150.04 |
7.27 ± 0.18 |
0.15 ± 0.01 |
8.09 ± 0.34 |
6.5 |
0.15 ± 0.04 |
5.79 ± 0.75 |
0.15 ± 0.10 |
5.91 ± 0.28 |
0.15 ± 0.04 |
6.92 ± 0.72 |
0.14 ± 0.04* |
8.09 ± 0.36 |
7.0 |
0.15 ± 0.02* |
6.03 ± 0.58 |
0.16 ± 0.10 |
5.35 ± 0.32 |
0.15 ± 0.04* |
7.32 ± 0.54 |
0.14 ± 0.08 |
8.17 ± 0.86 |
7.5 |
0.15 ± 0.06 |
5.94 ± 0.71 |
0.16 ± 0.01 |
6.17 ± 0.48 |
0.16 ± 0.02 |
7.43 ± 0.92 |
0.16 ± 0.04 |
7.73 ± 0.43 |
8.0 |
0.15 ± 0.06 |
5.99 ± 0.78 |
0.15 ± 0.06 |
6.05 ± 0.48 |
0.15 ± 0.06 |
7.45 ± 0.238 |
0.14 ± 0.04 |
7.87 ± 0.56 |
8.5 |
0.15 ± 0.02 |
5.66 ± 0.62 |
0.15 ± 0.04 |
6.21 ± 0.46 |
0.15 ± 0.04 |
7.55 ± 0.60 |
0.14 ± 0.02* |
7.97 ± 0.94 |
9.0 |
0.15 ± 0.14 |
5.29 ± 0.64 |
0.15 ± 0.02 |
5.91 ± 0.28 |
0.15 ± 0.08 |
7.32 ± 0.54* |
0.14 ± 0.06 |
8.34 ± 0.94 |
10.0 |
0.15 ± 0.06 |
5.25 ± 0.84 |
0.15 ± 0.12 |
6.14 ± 0.98 |
0.15 ± 0.04 |
7.56 ± 0.24 |
0.13 ± 0.08 |
8.39 ± 0.68 |
|
Note: All values are mean and standard deviation of three replicates.
*Significant (p<0.01) |
Table 4: Enzymatic Activities (Umg/prot) of Superoxide Dismutase (SOD) and Lignin peroxidase (LPX) in earthworm tissue after the exposure of different Zinc oxide NPs. |