Purificationstep
Totalprotein(mg)
Totalactivity(Units)
Specificactivity(U/mg)
Purificationfold
%Yield
Celllysate
1871
929
0.49
1
100
80%(NH
4
)
2
SO
4
precipitation
743
655
0.88
1.8
71
SephadexG-100sizeexclusionchromatography
Table 1:
Purification summary of azoreductase from B. badius.
