Sr.No Cofactor Km(µM) Vmax(U/mgprotein)
1 NADH 1.02 ± 0.07 16.5 ± 2.05
2 NADPH 6.12 ± 0.35 13.2 ± 1.2
3 FADH2 4.71 ± 0.81 10.8 ± 1.75
4 FMNH2 4.14 ± 0.76 10.3 ± 1.42
The Km and Vmax values were determined by Lineweaver-Burk plot. ± is the standard deviation of the mean of three independent experiments.
Table 2a: Cofactor requirement for azoreductase activity.