Sr.No
Cofactor
Km
(µM)
Vmax
(U/mgprotein)
1
NADH
1.02 ± 0.07
16.5 ± 2.05
2
NADPH
6.12 ± 0.35
13.2 ± 1.2
3
FADH2
4.71 ± 0.81
10.8 ± 1.75
4
FMNH2
4.14 ± 0.76
10.3 ± 1.42
The Km and Vmax values were determined by Lineweaver-Burk plot. ± is the standard deviation of the mean of three independent experiments.
Table 2a:
Cofactor requirement for azoreductase activity.
