Soil enzyme Buffer set at pH 5.5, pH 7 and pH 8.5 Substrates References
Acid/Alkaline phosphatase Acid phosphate buffer/ sodium orthophosphate buffer containing NaN3 (sodium azide) 25mM p-nitrophenyl phosphate oxidation [17]
β-Glucosidase 50mM phosphate buffer 1mM of p-nitrophenyl-β-D-glucopiranoside (ONPG) [22]
L-arginine ammonification 0.5ml of an arginine solution (0.2%) L-arginine [23]
Manganese peroxidase (MnP) 100µMol succinate lactate buffer (adjusted at pH 5.5, 7 and 8.5) 0.1µM of 3-methyl-2-benzthaiazolinone hydrazone hydrochloride (MBTH), 5µl of 3-dimethylaminobenzoic acid (DMAB), 0.2µM of MnSO4and 0.1µM H2O2 [24]
Lignin peroxidase (LiP) 50mM sodium acetate buffer along with 1% polyvinylpolpyrrolidone 0.1µM of 3-methyl-2-benzthaiazolinone hydrazone hydrochloride (MBTH), 5µl of 3-dimethylaminobenzoic acid (DMAB), 0.2µM of MnSO4 procedure followed byfew modifications by D’Annibale et al. [22]
Laccase enzyme Buffer A containing (0.1M sodium acetate, 0.005 M CaCl2, 0.05% tween 80 and 1% polyvinylpolpyrolidone 1mM of ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)  
Table 2: Soil enzyme assay.