Basal salt (g L-1) |
NaCl |
1.17 |
MgCl2·6H2O |
0.40 |
KCl |
0.30 |
CaCl2·2H2O |
0.15 |
NH4Cl |
0.27 |
KH2PO4 |
0.20 |
Electron acceptor and carbon sources (mmol L-1) |
Ferric citrate |
20 |
Lactate |
10 |
Acetate |
10 |
pH buffer (mmol L-1) |
Bicarbonate |
25 |
Vitamin solution (g L-1) |
Biotin |
2.0 x 10-4 |
Folic acid |
2.0 × 10-4 |
Pyridoxine-HCl |
10-3 |
Thiamine-HCl‧2H2O |
5.0 × 10-4 |
Riboflavin |
5.0 × 10-4 |
Nicotinic acid |
5.0 × 10-4 |
D-Ca-pantothenate |
5.0 × 10-4 |
Vitamin B12 |
10-5 |
p-Aminobenzoic acid |
5.0 × 10-4 |
Lipoic acid |
5.0 × 10-4 |
Trace metals(g L-1) |
Nitrilotriacetic acid |
1.5 × 10-4 |
MnCl2‧4 H2O |
5.2 × 10-3 |
CoSO4‧7 H2O |
1.8 × 10-3 |
NiCl2‧6 H2O |
2.0 × 10-4 |
CuSO4‧5 H2O |
10-4 |
ZnSO4‧7 H2O |
2.0 × 10-3 |
Na2MoO4‧2 H2O |
3.0 × 10-4 |
Na2SeO4 |
3.0 × 10-4 |
AlK(SO4)2‧12 H2O |
3.0 × 10-4 |
H3BO3 |
10-4 |
Redox indicator (1 mL : 1 L) |
Resozurin |
1 |
|