Environmental factor Optimum conditions Conditions required for microbial activity
Available soil moisture 25-28% water holding capacity 25-28% water holding capacity
 Oxygen >0.2 mg/ L DO, > 10 % air filled pore space for aerobic degradation Aerobic , minimum air filled pore space of 10%
 Redox potential  Eh > 50 mill volts  
Nutrients C:N:P= 120:10:1 molar ratio N and P for microbial growth
pH 6.5-8.0 5.5 to 8.5
Temperature 20-30 degree Celsius 15-45 degree Celsius
Contaminants Hydrocarbon 5-10 % of dry weight of soil Not too toxic
Heavy metals 700ppm Total content 2000ppm.
Table 4: Showing environmental conditions [57].