Figure 4: HPLC analysis of RB5 in a combined facultative anaerobic- aerobic treatment (a) sulphanilic acid standard (b) initial stage before treatment of RB 5 at t=0 (c) metabolites resulting from decolourisation at t=24 h (d) after 48 h incubation under facultative anaerobic and (e) after 48 h of incubation under aerobic condition.