Figure 5: Cadmium and pyrene toxicity evaluated based on fluorescence microscopy using selective live/dead staining. The fungus was grown and treated as described under Figure 3 legend. The nuclear stain DAPI stained both live and dead filaments, while PI stained only dead filaments. A-B: Control filaments stained with DAPI and PI, respectively; C-D: Cd (0.5 mM) - treated filaments stained with DAPI and PI, respectively; E-F: Pyr (10 ppm)- treated filaments stained with DAPI and PI, respectively; F-H: Cd (0.5 mM)+Pyr (10 ppm)-treated filaments stained with DAPI and PI, respectively.