Figure 5: Effect of addition of increasing concentrations of diamide (8.25-57 μmol/L) to a fixed GSH concentration (100μmol/L). The TNB, that absorbs 412 nm was produced when the remaining GSH were reduced by DTNB. All reagents were dissolved in 0.1 mol/L phosphate buffer (pH 7.5). Error bars denote standard deviation from 5 independent experiments.