Figure 3: Fluorescence emission spectra of immobilized BSA in acetate buffer (pH 3.8). 3.75 mg of Anionic PP and BSA were added to acetate buffer solution (pH 3.8) and incubated for 24 h at 4°C. BSA concentrations were 0.4, 0.6, 0.8, and 1.0 μM, respectively. Immobilized BSA was washed and suspended with acetate buffer. The adsorption and immobilization amounts of BSA against anionic PP were (a) 4.73, (b) 8.96, (c) 13.8 and (d) 18.2 μg (mg-particle)–1. Fluorescence excitation was took place at 295 nm.