Circadian
Figure 2: Circadian rhythms with star-shaped initial conditions in an AtCCA1::LUC lettuce leaf induced by spatiotemporal illumination using an LCD projector. (a) Bright and dark star pattern illumination. (b) Bioluminescence images: the star region was at subject dusk (left) and subject dawn (right). (Scale bar: 10 mm). (c) Snapshots of bioluminescence under DD. (Interval between images = 4 h, scale bar = 10 mm) (d) Phase images obtained from bioluminescence images of the leaf in Figure 2c. (Interval between images = 4 h) (e) Inversion phase images of the leaf of Figure 2c. t = 31 (left) and 43 h (right), respectively. (f) Normalized bioluminescence in the star region (black) and the region to its right (white), indicated by the solid line (A) and dashed-dotted line (B) in Figure 2e. There were disturbances in t = 16 to 21. Black and white triangles indicated measured peak time of the normalized bioluminescence and arrows indicated expected peak time.Black bars: subjective night; hatched bars: subjective day. The lines were used only to guide the eye.