Figure 2: Analysis of purity of CYP27B1, ADX and ADR(A) SDS/PAGE of CYP27B1 (lane 2), ADX (lane 3), and ADR (lane 4) after nickel affinity chromatography. (B) Western blot of CYP27B1 (lane 2), ADX (lane 3), and ADR (lane 4) after nickel affinity chromatography. Lane 1 (leftmost one in both panels) is the protein standard.