Initial broth volume in the fermentor (L) Feed rate glycerol
(g/h )
Pulse feeding times
(h )
1.5
1.5
1.5
1.5
1.5
2.5
3.5
4.5
5
10
12
12
12
12
12
1.5
1.5
2.5
10
17
17
Table 1: Experimental protocol of the various strategies of fed batch fermentations.