Figure 1: Germination of Hemileia vastatrix urediniospore after storing at different temperatures a, b, c: Germination of fresh urediniospores cultured for 8h,(a) 12h,(b) and 16h(c) on water agar. Swollen appresorium like structure is seen at the end of the germ tube. (D) Several viable spores have germinated after storing at -80°C for one year indicating good viability. (E) Non-viable spore.