| Primer name |
Conserved amino acid motif |
Forward Reverse |
Primer sequence |
| Non-degenerate primers designed on the basis of the first coffee RGAs isolated (Noir et al. 2001). |
| Ploop-Cof |
GVGKTT |
F |
5’-GGGGGTGGGGAAGACGACTC-3’ |
| GLPL-Cof |
DGLPLAL |
R |
5’-AGGGCGAGGGGGAGGCCATC-3’ |
| Degenerate primers first set designed on the basis (Noir et al. 2001). |
| Ploop1 |
GGV/I/MGKTT |
F |
5’-GGIGGIGTIGGIAARACNAC-3’ |
| Ploop2 |
GGV/I/MGKTT |
F |
5’-GGNGGNRTNGGNAAAACAAC-3’ |
| Ploop4 |
GGV/I/MGKTT |
F |
5’-GGNGGNRTNGGNAARACTAC-3’ |
| Ploop5 |
GGV/I/MGKTT |
F |
5’-GGNGGNRTNGGNAARACCAC-3’ |
| GLPL1 |
GL/FPL/FAL/V |
R |
5’-IARIGCIARIGGIARNCC-3’ |
| GLPL2 |
GL/FPL/FAL/V |
R |
5’-CAHHGCNAAHGGHAAHCC-3’ |
| GLPL3 |
GL/FPL/FAL/V |
R |
5’-CAANGCCAANGGCAANCC-3’ |
| GLPL4 |
GL/FPL/FAL/V |
R |
5’-CAGNGCNAGNGGNAGNCC-3’ |
| Degenerate primers second set on the basis (Chen et al. 2007) |
| F1 |
Ploop (GGV/I/MGKTT) |
F |
5’-GGHDYVGGKAARACWAC-3’ |
| F2 |
Ploop (GMGGV/I/SGKTT) |
F |
5’-GGDATGVSVGGHDYVGGKAARAC-3’ |
| F3 |
Ploop (GGV/I/MGKTT) |
F |
5’-GGNGGNRTHGGNAARACHAC-3’ |
| F4 |
Ploop (GGV/I/MGKTT) |
F |
5’-GGNGTNGGNAARACNAC-3’ |
| F5 |
Ploop (GGV/I/MGKTT) |
F |
5’-GGNGGNGTNGGNAANACNAC-3’ |
| F6 |
Ploop (GGV/I/MGKTT) |
F |
5’-GGNGGNRTNGGNAARACNAC-3’ |
| HDR1 |
Hydrophobic domain (GL/FPL/FA/VL) |
R |
5’-AKWGCYARRGGDARYCC-3’ |
| HDR2 |
Hydrophobic domain (GL/FPL/FA/VL) |
R |
5’-GCMRCCARAGGMARYCC-3’ |
| HDR3 |
Hydrophobic domain (GL/FPL/FA/VL) |
R |
5’-AGNGCHAGNGGYAANCC-3’ |
| HDR4 |
Hydrophobic domain (GL/FPL/FA/VL) |
R |
5’-AANGCHAGNGGYAANCC-3’ |
| HDR5 |
Hydrophobic domain (GL/FPL/FA/VL) |
R |
5’-AGIGCHAGNGGNAGNCC-3’ |
| HDR6 |
Hydrophobic domain (GL/FPL/FA/VL) |
R |
5’-ARNWYYTTVARDGCVARWGGVARWCC-3’ |
