S.No RGA Clone name CBC Insert size (bp) NCBI BLAST best hit   Score E value Accession No. Details of hit 1 CBC 7 500 924 0.0 AJ298884.1 Coffea arabica partial ORF for disease resistance-like protein 2 CBC8 340 182 1e-42 AJ308824.1 Coffea arabica microsatellite DNA 3 CBC139 486 41.0 5.5 AJ298884.1 Coffea arabica partial ORF for disease resistance-like protein 4 CBC140 528 926 0.0 AY606825.1 Coffea arabica clone P5RANDF disease resistance-like protein gene 5 CBC141 523 911 0.0 AJ298884.1 Coffea arabica partial ORF for disease resistance-like protein 6 CBC142 527 911 0.0 AY606825.1 Coffea arabica clone P5RANDF disease resistance-like protein 7 CBC143 486 41.0 5.5 AJ298884.1 Coffea arabica partial ORF for disease resistance-like protein

Total RGAs isolated from genomic DNA using Ploop-Cof and GLPL-Cof and sequenced = 7 Number of clones sequence matches with disease resistance-like protein = 6 Number of clones sequence not matches with disease resistance-like protein = 1

Table 3: Details of NCBI BLASTN sequence results of RGAs isolated from genomic DNA using non degenerate primers.