S.No RGA Clone name CBC Insert size (bp) NCBI BLAST best hit Score E value Accession No. Details of hit 1 CBC183 514 857 0.0 EF566662.1   Coffea spp. RGA putative NBS domain resistance protein gene 2 CBC184 592       No Hit 3 CBC185 528 867 0.0 EF566686.1 Coffea spp. RGA putative NBS domain resistance protein gene 4 CBC186 525 852 0.0 AJ298884.1 Coffea arabica partial ORF for disease resistance-like protein 5 CBC187 507 144 4e-36 ABU51609.1 Putative NBS domain resistance protein [Coffea spp.] 6 CBC188 531 880 0.0 EF566686.1   Coffea spp. RGA putative NBS domain resistance protein gene 7 CBC189 530 880 0.0 EF566686.1 Coffea spp. RGA putative NBS domain resistance protein gene 8 CBC190 454       No Hit 9 CBC191 530 880 0.0 EF566686.1 Coffea spp. RGA putative NBS domain resistance protein gene 10 CBC192 600       No Hit 11 CBC193 527 915 0.0 AJ298884.1 Coffea arabica partial ORF for disease resistance-like protein,

Total RGAs isolated from genomic DNA using Ploop-4 and GLPL-4 and sequenced = 11 Number of clones sequence matches with disease resistance-like protein =8 Number of clones sequence not have significant match = 3

Table 4: Details of NCBI BLASTN sequence results of RGAs isolated from genomic DNA using degenerate primer.