Figure 3: mce4A siRNA molecular beacon interacts with its target in M. smegmatis. An overnight culture of M. smegmatis MC2155 was used to inoculate 7H9 broth and grown to an A600 of 0.3. Mce4 siRNA molecular beacon or random oligonucleotide molecular beacon was added at 10μM to the M. smegmatis, incubated at 37°C for 5hrs and imaged using a confocal microscope.