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Figure 4: Fluorescence measurement in M. smegmatis infected U937 cells. Non-infected or Ms-infected U937 macrophages were either not transfected (pink) or transfected with random oligonucleotide (purple) or mce4A siRNA (green) molecular beacon at 1 or 10μM followed by fluorescence measurement after 3hr incubation. The effect of molecular beacon transfection on fluorescence intensity was compared between transfected and non-transfected samples using ANOVA. n=32; *, +p≤0.001 |