| Pre-Cold treatment time |
Callus formation rate(%)
Rhyncostylisretusa |
Remarks |
| 0hrs |
- |
Anthers failed to respond & become necrotic . |
| 8hrs |
- |
Anthers failed to respond & become necrotic . |
| 24hrs |
12.5% |
Friable, acholophyllous and organogenetic callus formed |
| 48hrs |
12.5% |
Anthers become necrotic within one week within one wk of culture. |
| 72hrs |
- |
Anthers failed to respond & become necrotic . |
