Plant
Figure 1: Plant regeneration from leaf and petiole explants of cabbage (Brassica oleracea L. var. capitata cv. Pride of India).
a. Callus formation obtained from leaf explants on medium supplemented with (MS+ 0.22mg/l TDZ + 0.02mg/l NAA).
b. Shoot regeneration from leaf explants on medium supplemented with (MS+ 0.22mg/l TDZ + 0.02mg/l NAA).
c. Callus formation obtained from petiole explants on medium supplemented with (MS+ 0.22mg/l TDZ + 0.02mg/l NAA).
d. Shoot regeneration from petiole explants on medium supplemented with (MS+ 0.22mg/l TDZ + 0.02mg/l NAA).
e. Root regeneration from the in vitro developed shoot regeneration from explants on the medium supplemented with (MS + 0.10mg/l NAA).
f. Fully developed plantlets of cabbage taken out of medium (after proper washing) after 15 days of culturing showing well developed root system. (Later on transferred to pots containing pre-sterilized cocopeat for acclimalization).
g. Regenerated plantlets transferred to plastic pots containing mixture of pre-sterilized cocopeat for hardening.
h. Plantlets transferred to potting mixture (containing sand + soil + FYM).