Figure 5: Coomassie blue stained SDS-PAGE pattern of purified PEGase1 (arrowed band) Lane M: Standard MW protein marker; lanes 1 and 2: the culture supernatant of the control and pegase1 gene-containing fungus; lane 3: condensed using ammonium sulfate (AS, 40 ~ 70% saturation); lane 4: purified PEGase1 using HiTrapTM CaptoTM Q gel.