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Figure 1: SDS-Page of extracted EPS: EPS contains numerous proteins with different apparent molecular weight (left lane); part of which are susceptible to cleavage by trypsin. Proteins after digestion with trypsin are shown in the second lane. After precipitation of proteins by trichloroacetic acid (TCA) the majority of proteins remained in the pellet (3. lane), and some were in the supernatant (4. lane). |