Figure 2: Serial transfers of 33C KO Library (knockout library) grown in RMA5 (35°C) shake flasks to enrich for faster growers. KO1 and KO2 were cultures revived from two vials of the frozen 33C KO library. 33C was included as a control in this enrichment growth. A, first transfer, B, third transfer, C, fifth transfer, D, ninth transfer, E, twelfth transfer, F, thirteenth transfer, G, fifteenth transfer and H, seventeenth transfer.