| Strain or plasmid |
Description |
Reference |
| Strain |
|
| Escherichia coli |
DH5α |
F– Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rK–,
mK+) phoAsupE44 λ– thi-1 gyrA96 relA1 |
Invitrogen |
| SCS110 |
rpsL (Str-r) thrleuendA thi-1 lacYgalKgalTara
tonAtsx dam dcm supE44 Δ(lac-proAB) [F′ traD36
proAB lacIqZΔM15] |
Agilent
Technologies |
| Zymomonasmobilis |
8b |
ZM4 integrated with Tc-PgapxylAB/Cm-Penotaltkt; glucose/xylose
utilizing |
our lab; [2] |
| Strain 33 |
8b integrated with loxP-aadA-loxP-Pgap-araBAD;
glucose/xylose/arabinose utilizing |
this study |
| 33C |
aadAfree derivative of Strain 33; glucose/xylose/arabinose utilizing |
this study |
| 13-1-17 |
isolate from 33C genomic KO library; glucose/xylose/arabinose utilizing |
this study |
| 15-1-34 |
isolate from 33C genomic KO library; glucose/xylose/arabinose utilizing |
this study |
| Plasmid |
pMOD2Sp |
Ampr,Spr; integrative plasmid containing ME-loxP-aadA-loxP-ME for
transposition and KO library construction; non-replicative in Z. mobilis |
this study |
| pMOD2Sploxara |
Ampr,Spr; integrative plasmid containing ME-loxP-aadA-loxP-PgaparaBAD-
ME for transposition; non-replicative in Z. mobilis |
this study |
