8b 33C 13-1-17 araBADin native plasmid (ref. 8b) no yes yes aadAin ZMO1650 (ref. 8b) no no yes araBmutation (ref. E .coli) no Yes: R127E,S128A, A490G Yes: R127E, S128A, A490V ZMO0353 mutation (ref. 8b) no no Yes: A376V ZMO1200 mutation (ref. 8b) no no yes

ref: referenced genome sequence ZMO1650: β--lactamase gene ZMO0353: 4--diphosphocytidyl--2C--methyl--D--erythritol synthase gene ZMO1200: ribose--5--phosphate isomerase Gene (rpiB)

Table 2: Transposon insertions and mutations identified through Next Generation Sequencing.