Oxygen producing approach Cell type Hypoxic parameters Effect References Oxygen producing scaffold NIH 3T3 fibroblasts hypoxicglovebox system (1% O2, 5% CO2) (BioSpherix, USA) Higher cells numbers found as compare to normoxic condition [11] Oxygen releasing microspheres Clone Neuro-2a (N2a, ATCC No. CCL-131) Artificially by closing the air passage of the culture dish Viability of the cells increased [12] Oxygen producing micro-system Skeletal muscle cells (L6) Billups–Rothenberg modular incubation chamber, USA (1% O2, 5% CO2, and 94% N2) Increase viability in the skeletal muscle cells [13]

Table 4: Hypoxic parameters and instrumentations used for the in-vitro experiments.