Figure 1: Quantitation of lamellipodia dynamics using computer-assisted stroboscopic analysis of cell dynamics (SACED). After cell adhesion, images are captured and the velocities of lamellipodia protrusion and retraction are calculated using a 10-pixel-wide (1.06 mm) box drawn in the direction of cell protrusion to create montages of images captured every 5 sec for 5 min. dt1:time to protrusion, dt2: time to retraction, dx: protrusion distance.