Figure 2: Relative EsTUBA expression levels (2−ΔΔCt) in Eriocheir sinensis larvae at different developmental stages. (A): Bars represent mean ± S.E. (n=3). Statistical significance is indicated with lowercase letters (Tukey’s post hoc test, α=0.05). (B): EsTUBA mRNA expression in larval development phase of E. sinensis. EsTUBA and β-actin mRNA expression in stage O (embryo stage), stage Z1 (the first zoeal stage), stage Z2 (the second zoeal stage), stage Z3 (the third zoeal stage), stage Z4 (the fourth zoeal stage), stage Z5 (the fifth zoeal stage), stage M (megalopa stage), stage J1 (the first juvenile crab stage), stage J2 (the second juvenile crab stage) and stage J3 (the third juvenile crab stage).