| Application |
Primer |
Primer sequence (5’→3’) |
Amplicon length (bp) |
| 5'-RACE |
5'RACE Abridged Anchor Primer
AUAP
UAP
5'RACE Anchor Primer |
5'-GGCCACGCGTCGACTAGTACGGGGGGGGGG-3'
CUACUACUACUAGGCCACGCGTCGACTAGTAC-3'
5'-CUACUACUACUAGGCCACGCGTCGACTAGTACGGGGGGGGGG-3' |
|
| |
EsTUBA-GSP1 |
5'-ATCTGGTTTGCGGGCT-3' |
1166 bp |
| |
EsTUBA-GSP2 |
5'-AAGCAGGCGTTGGTGATCTCA-3' |
1148 bp |
| |
EsTUBA-GSP3 |
5'-GATAACGGGAGCGTAGGTCAC-3' |
1089 bp |
| 3'-RACE |
3'-RACE 10 × Universal Primer A Mix (UPM) |
5'-CTAATACGACTCACTATAGGGCAAGCAGTGGTATCAACGCAGAGT-3'
5'-CTAATACGACTCACTATAGGGC-3' |
|
| |
EsTUBA-31
EsTUBA-32 |
5'-CATCCACTTCCCTCTGGTGACCTACGCT-3'
5'-GCTGTCTGTGTCTGAGATCACCAACGCC-3' |
643 bp
580 bp |
ORF full-length confirmation
semi-quantitative RT-PCR
Real-time qPCR |
QC304F
QC304R |
5'-CAAAATGCGTGAGTGCATCTC-3'
5'-GATTTAATACTCCTCTCCCTC-3' |
1363 bp |
EsTUBAF
EsTUBAR
β-actin-F
β-actin-R
QEsTUBA-F
QEsTUBA-R
Qβ-actin-F
Qβ-actin-R |
5'-TCACTGCCTCCCTGAGATTC-3'
5'-AATGGTCCTCTTGGTCTTGATG-3'
5'-CCGTGACCTCACAGACTACCT-3'
5'-CGGTGGTCGTGAAGGTGTAG-3'
5'-GATCGTGTCCTCCATCACTG-3'
5'-GTCACCAGAGGGAAGTGGAT-3'
5'-CTCCTGCTTGCTGATCCACATC-3'
5'-GCATCCACGAGACCACTTACA-3' |
311 bp
65 bp
114 bp |
