Figure 2: Time profiles of VEGF release from gelatin hydrogel sheets incorporating 3 ng of VEGF in Matrigel. (A) Schematic illustration of 2-directional diffusion patterns of VEGF from gelatin hydrogel sheet incorporating VEGF in Matrigel. (B) Amount of VEGF in the Z-axis direction of Matrigel from the gelatin hydrogel sheet. The incubation time was 15 (○) and 30 min (ϒ) or 1 (□), 3 (●), and 8 hr (▲).