Parameters First Stage (early stationary phase) Second Stage (late stationary phase) Total time (d) Biomass concentration (g.L-1) µ (d-1) (exponential phase) Biomass productivity (g.L-1.d-1) Lipid content (%) Lipid productivity (mg.L-1.d-1) TAG content (% in total lipids) 13 0.485 0.151 0.032 ± 0.001 19.6 ± 0.6 6.7 ± 0.2 27.7 ± 1.1 17 0.488 - 0.027 ± 0.004 25.4 ± 0.4 7.5 ± 0.1 41.3 ± 0.1

Table 1: Comparative analysis between the first and second stage cultivation. The productivity data are reported as mean ± standard deviation of triplicates.