Biomarker Detection/Application Analytical Tools
Pigments Presence of photosynthesisSpecificantioxidants, Vit A Fluorescence, HPLC, Mass spectra
CytochromeP4501A  Presence of organic contaminationcertain PAHs, PCBs, PCDDs, etc. Enzyme activity (fluorometry andspectroscopy) and amount (Elisa)
DNA—damage Presence/damage of organicxeno-biotics (PAHs, PCBs and PCDDs)    DNA strand breaks measurement,
AChE inhibition Indicator of exposure to organo-phosphorous Carbamate, toxins,metals (Cd, Pb, Cu, etc.,). Enzyme activity (spectrophometer pH)
Metallothioneins Indicator of exposure to metals(Zn, Cu, Cd and Hg).In: vertebrates (mammals) andAndinvertebrates (molluscs, fish)Algae. Proteins (pulse polarography, liquidchromatography
Table 3: Particular molecular biomarkers of biological effects of contaminants on aquatic organisms and respective analytical technique of their measurements (modified after [2]).