Total proteins from the untreated control (lane1) and cells treated with 1 mM, 4 mM, 6 mM and 10 mM (lane 2-5 respectively) of BA were separated on 10% SDS-PAGE and transferred into nitrocellulose membrane, and was probed with anti-PAR as shown in upper panel. The blot was stripped and re-probed with beta actin as shown in lower panel.
