Figure 3: Expression of canine interferon - γ in silkworm and its gene modification (A) Comparison of productivity: Productivity of canine interferon- γ in silkworm-baculovirus system is higher than any other protein expression system. (B) Gene modification of N-glycosylation sites: Asn at position 16 and 83 is changed to Gln to remove N-glycosylation site. (C) Western blots analysis: Lane 1, unchanged (glycosylated) canine interferon - γ; Lane 2, genetically changed (nonglycosylated) canine interferon - γ. Recombinant proteins showed at least four visible bands before modification. After removing N-glycosylation site, the recombinant protein is expressed in one visible band.