Classification Name Method Tissue Tag Yield Ligand-binding affinity (value from other hosts) Reference Immunoreceptor IL4-Rα virus haemolymph Fc 0.19 mg/ml haemolymph N.D. (Binding activity was confirmed by gel filtration analysis) [26] IL13-Rα virus haemolymph Fc 0.014 mg/ml haemolymph Kd = 2.3 nM (≈ 4 nM, CHO cells [29]) [26] KIR2DL1 bacmid haemolymph 6xHis ~0.2 mg/larva Kd = 8.6 ± 0.69 μM (33 μM, E .coli [32]) [17] FasR virus haemolymph Fc 22.5 mg/26 ml haemolymph N.D. (Binding activity was confirmed by immunoprecipitation  and size exclusion chromatography) [39] FasR virus haemolymph Fc 13.5 mg/25 ml haemolymph N.D. (Binding activity was confirmed by size-exclusion chromatography) [40] GPCR u-opioid receptor transgenic fat body 6xHis 150–250 ng/larva* Kd = 1.4~2.1 nM (0.37 ± 0.09 nM, Sf9 [55]) [54] nociceptin receptor bacmid fat body 6xHis N.D. EC50 = 9.3~24 nM (12 nM, Sf9 [62]) [56]

Table 1: Human immunoreceptors and GPCRs expression using silkworm.