Run pH Lactose (g/l) Yeast cells
concentration (Y) (g/l)
Temperature
(
°C)
Response
Function (RF)
1 5 100 0.6 34 1.35
2 5 20 0.6 34 1.55
3 4 60 0.6 26 1.47
4 4 40 0.4 30 1.36
5 5 80 0.4 30 1.05
6 6 60 0.6 42 0.85
7 6 40 0.8 38 1.17
8 3 80 0.8 38 1.11
9 7 60 0.6 34 0.90
10 4 60 0.6 34 0.98
11 4 40 0.4 38 1.05
12 6 80 0.4 38 0.75
13 6 40 0.8 30 1.48
14 5 80 0.8 30 1.42
15 5 60 0.2 34 0.75
16 6 60 1.0 34 0.85
17 6 40 0.4 30 1.50
18 6 40 0.4 38 1.20
19 6 80 0.4 30 1.45
20 4 80 0.4 38 1.05
21 4 40 0.8 30 1.08
22 4 40 0.8 38 0.75
23 4 80 0.8 30 1.03
24 5 80 0.8 38 0.73
25 5 60 0.6 34 1.59
Table 1: Experimental design and CCD results of response surface methodology.