Sample Type Wavelength, λ(Å) Peak Values Absorbance, ā Peak Values M-TB Positive M-TB Negative M-TB/HIV Co-infected M-TB/HIV Uninfected Mean Values   Sputum   320 0.2918 – 0.7877 --- --- --- 0.4588 ± 0.1468 --- 0.2657 – 1.2501 --- --- 0.6244 ± 0.3545   290 --- --- 0.0231 – 0.0498 --- 0.0379 ± 0.0108     --- 0.2657 – 1.2501 0.6244 ± 0.3545   Macrophage   320 0.0206 – 0.0736   --- --- 0.0496 ± 0.0116 --- 0.0478 – 0.1148 --- --- 0.0784 ± 0.0206   290 --- --- 0.0152 – 0.0637 --- 0.0456 ± 0.0106 --- --- --- 0.0478 – 0.1148 0.0784 ± 0.0206

Table 1: Comparison between Peak Absorbance values of M-TB Positive, M-TB/HIV Positive and Negative Sputum Components respectively.