No. Primer name PCR Codition Product Size (bp)
Annealing Mg Conc. dNTPs Cycle Number
( OC) (mM) (mM) (cycles)
1 DTLSH 7 60 2 200 40 131
2 DTLSH 8 60 2 200 40 174
3 DTLSH 9 60 2 200 40 210
4 DTLSH 12 60 2 200 40 193 / 205
Table 2: PCR reduction component and primer qualities.
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