Variable AB TC EC Coefficient P-value Coefficiente P-value Coefficiente P-value Intercept 1.83e5 <2e-16 5.92e2 <2e-16 0.34 0.15 Season 1.1 0.66 2.48 5.8e-6 8.88 1.6e-15 Tank 0.29 0.011 2.39 0.17 0.19 0.065 Season*tank 1.0 0.87 0.56 0.012 0.91 0.78

Table 1: Coefficients and significance level of the significant variables applying the negative binomial model for the quantification analysis of AB, TC and EC bacteria throughout the O. niloticus production chain.