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Figure 2: PCR products obtained by PCR using F. psychrophilum-specific primer set from EMA treated F.psychrophilum cells. |
The cells had been previously exposed 20 min to hydrogen peroxide 250, 500, 1,000 or 2,000 ppm (250-2,000, respectively) or not-exposed (0). Positive and negative controls were EMA-untreated cells (-EMA) and 95°C-heated cells (95°C), respectively. F.psychrophilum strains were the type-strain JIP02/86 and five other strains freshly isolated from five French farms. |