Chemical products Concentration (ppm) Viability1
CPM2 % (mean ± SE) RPS3 (%)
Control (PBS)   11.36a ± 2.64 0.00
Bronopol 50 11.10a ± 3.60 2.3
  500 10.50a ± 2.82 7.5
  2,000 8.50a ± 3.56 25.2
Hydrogen peroxide 40 9.60a ± 2.77 15.5
  1,000 8.30a ± 2.14 26.9
  2,500 10.10a ± 3.12 11.1
Glutaraldehyde 300 10.30a ± 4.46 9.3
  1,500 27.30a ± 13.83 -140.4
  2,000 44.50b ± 15.83 -291.8
Incimaxx Aquatic® 10 11.60a ± 5.15 -2.1
  150 12.60a ± 4.98 -10.9
  185 14.30a ± 4.04 -25.9
Chloramine-T 50 14.10a ± 5.85 -24.2
  600 9.40a ± 3.75 17.2
  800 8.50a ± 2.95 25.2
1Rainbow trout eggs (5 days before hatching) were treated 20 minutes with the corresponding chemical product and viability of eggs/fry was recorded over a 5-weeks period.
2CPM, the cumulative percentage mortality (n=5 per treatment).
3RPS, the relative percentage survival; it was determined relative to PBS treatment.
Mean CPM values with different superscripts indicate significant difference at p<0.05.
Table 3: Impact of chemical treatments on rainbow trout eggs/fry viability.