Superantigenic toxin type Sequence (5' to 3') for Forward/Reverse primers Ta/°C product size SEA GCAGGGAACAGCTTTAGGC GTTCTGTAGAAGTATGAAACACG 53 521bp SEB GTATGATGATAATCATGTATCAGCAATA CGTAAGATAAACTTCAATCTTCACATC 53 625bp SEC GAGTCAACCAGACCCTATGCC CGCCTGGTGCAGGCATC 55 614bp SED GAGACTAGCCGCAATCTATCC GCTGCATTTAGTAATGCTGGCTG 54 650bp SEE GGTAGCGAGAAAAGCGAAG GCCTTGCCTGAAGATCTAGCTC 54 450bp SEG TGAATGCTCAACCCGATCCTAAAT CAAACCAAAAACTTGTATTGTTCTTTTCA 54 580bp SEH TTCACATCATATGCGAAAGCAGAA CAGATTTTAAAGTTTTATTGTCTTCA 50 625bp SEI CGTATGCTCAAGGTGATATTGGTG AAAAACTTACAGGCAGTCCATCTCC 55 580bp SElJ TTTAGGATCCCTACAGAACCAAAGG GTTTCCATGGATAGCAAAAATGAAAC 50 750bp SElK GTGTCTCTAATAATGCCAGCGCTC TTTGGTAGCCCATCATCTCC 55 650bp SElL CACCAGAATCACACCGCTTA TCCCCTTATCAAAACCGCTAT 51 450bp SElM TTTTGCTATTCGCAAAATCATATCGCA TCAACTTTCGTCCTTATAAGATATTTCTAC 54 687bp SElN TGAGATTGTTCTACATAGCTGCAA AATTAGATGAGCTAACTGTTCTATTATCAC 54 720bp SElO TAGTGTAAACAATGCATATGCAAATG ATTATGTAAATAAATAAACATCAATATGATA 50 721bp SElP GGAAGCTAAAGCAGAGACAC CCCGTTTCATATGAAGTGCCACC 51 660bp SElQ GCTTCAAGGAGTTAGTTCTGG CTCTCTGCTTGACCAGTTCCGGTG 57 510bp TSST-1 GAAATTTTTCATCGTAAGCCCTTTGTTG TTCATCAATATTTATAGGTGGTTTTTCA 53 625bp

Table 1: Base sequences, annealing temperature selected for the primer set (Ta), and product size of amplified products and the control for the superantigen primer sets.