Figure 1: (Crystal violet, x400) Microphotography of the cell migration assay. Cells (5x103) were seeded in the upper compartment of transwell culture chambers (Falcon Cell Culture Inserts, Becton-Dickinson Laboratories, Orangeburg, NY, USA) in 24-well microplates and cultured in 1% FCS medium with 1000 IU/mL of MF. After 48 hours, melanoma cells migrated to the filter (8 μm diameter pores) and adhered to the lower surface of the 24-well microplate.