Multiferon (U/ml)

Clonogenic ability Number of colonies

Migratory ability (area, arbitrary units)

0

29,5 ± 3,5

37,2 ± 3,5

500

31,0 ± 0,5

nd

1000

30,4 ± 6,7

35,7 ± 0,2

Table 1: Effect of Multiferon on clonogenic and migratory ability of B16F10 melanoma cells. The number of colonies was counted under microscope and values were expressed as the mean ± SD. In the migratory assay, positive area for crystal violet stained cells were analysed using Image J software. Values are expressed as the cell stained areas in arbitrary units ± SD of at least three independent experiments performed in quadruplicate.