Protein Abbreviation Molecular Weigth [Da] IP [pH] NCBI Prot-no UniProtKB / Swiss-Prot. Protein name coHaCaT
Metabolism
AHCY 47,716  5.82 GI:20141702 P23526 Adenosylhomocysteinase ↑
ENO1 47,169 7.01 GI:119339 P06733 Alpha-Enolase -
ASS1 46,530 7.15 GI:20141195 P00966.2 Argininosuccinate synthase -
GSTP1 23,356 5.43 GI:121746 P09211.2 Gluthadion S-transferase P -
GAPDH 36,053 8.57   GI:120649 P04406 Glyceraldehyde-3-phosphate dehydrogenase NM
NADP 46,659   6.53 GI:21903432 O75874 Isocitratedehydrogenase -
LDH-A 36,689 8.44 GI:126047 P00338 L-Lactate dehydrogenase A chain ↑
PPIA 18,012 7.68 GI:51702775 P62937 Peptidyl-prolylcis-trans isomearase A  ↓
PRDX1 22,110   8.27 GI:548453 Q06830 Peroxiredoxin-1 ↑
PRDX2 21,892    5.66 GI:2507169 P32119 Peroxiredoxin-2  ↓
PRDX5 22,086 8.93 GI:317373539 P30044 Peroxiredoxin-5  ↓↓
PRDX6 25.035 6.0 GI:1718024 P30041 Peroxiredoxin-6 -
PGAM1 28,804   6.67 GI:130348 P18669 Phosphoglyceratemutase 1  ↓↓
PGK1 44,615 8.3 GI:52788229 P00558 Phosphoglycerate kinase 1 ↑
PSMB2 22,836 6.49 GI:28559000 P49721 Proteasome subunit beta type 2  ↓
TPI1 30,791 5.65 GI:353526311 P60174 Triosephophateisomerase 1  ↓
Cytoskeleton and cell membrane compartments
ANXA1 38,714 6.57   GI:113944 P04083 Annexin A1  ↓
ANXA2 38,604 7.57 GI:113950 P07355 Annexin A2 -
ANXA5 35,937 4.94 
GI:113960
 P08758 Annexin A5 -
CLIM1 36,072 6.56 
GI:20178312
 O00151 PDZ and LIM domain protein 1  ↓
PFN1 15,054 8.44 
GI:130979
 P07737 Profilin-1 ↑
14-3-3 proteins
14-3-3e 29,174 4,63 GI:51702210 P62258 14-3-3 protein epsilon ↑
14-3-3s 27,774 4,68 GI:398953 P31947 14-3-3 protein sigma ↑↑
14-3-3z 27,745 4,73 GI:52000887 P63104 14-3-3 protein zeta/delta -
Others
maspin 42,100 5.72 GI:229462757 P36952 Maspin ↑
RKIP 21,057 7.01 
GI:1352726
 P30086 Phosphatidylethanolamine-binding protein 1 -
PCBP1 37,498 6.66 GI:42560548 Q15365 Poly (rc)-bindingprotein 1 ↓
TAGLN2 22,391 8.41 GI:586000 P37802 Transgelin-2 -
Table 1: Identification of proteins with consistent expression in HaCaT and coHaCaT cells. GAPDH was used as normalization marker (NM) and the pure cultured HaCaTs were set 100%. The results for coHaCaTs were classified in ↑ (over-expression of 25-50%), ↑↑ (over-expression of >50%), ↓ (down-regulation of 25-50%) and ↓↓ (downregulation of >50%).