Cell survival measured through MTT assays following incubation of (a) GM05659 fibroblasts; (b) p53-mutant HaCaT keratinocytes, (c) neonatal foreskin primary
keratinocytes and, (d) squamous cell carcinoma cell lines with voriconazole or its N-oxide metabolite given UVB pre- or post-incubation (n=6) compared with the
methanol and ethanol solvents, respectively (n=6); and, the caffeine and ABT-888 positive controls (n=12). 100% cell survival is indicated by an orange line for each
graph. The drug-incubated cells were UVB-irradiated using 6 x BLE8T312 fluorescent lamps. Peak UV emission was seen at 310 nm and 50% intensity of the emitted
wavelengths ranged between 295-325 nm as measured via a UVB-Dosimeter (SpectrolineCorporation™). Error bars are calculated based on the standard error of the
mean. |
